James Stuart Blackton (oft J. Stuart Blackton; * 5. Januar 1875 in Sheffield, Yorkshire; † 13. August 1941 in Hollywood, Kalifornien) war ein britisch-US-amerikanischer Karikaturist, Filmproduzent, Regisseur und Pionier des Animationsfilms. ()
James Stuart Blackton was a pioneer Anglo-American filmmaker and can be credited with the first feature-length American production. He founded Vitagraph Studios in 1897 and was among the first filmmakers to use stop-motion and drawn animation.